Dna Gel Electrophorosis Essay, Research Paper
Introduction:
DNA, Deoxyribonucleic acid, is a double stranded, helical nucleic
acid molecule which determines inherited structure of a protein. The
?steps? are made of bases: adenine, guanine, cytosine, and thymine. The
sides are sugar and phosphate molecules. Restriction enzymes are
enzymes that cut DNA at restriction sites, leaving fragments blunt or
sticky. The restriction fragments are separated using a technique called
gel electrophoresis.
DNA has a negative charge so when an electrical charge is
applied it makes DNA move to the positive side. DNA is placed in
agarose gel. Smaller fragments move faster. The purpose of this lab is to
separate DNA fragments using gel electrophoresis. Hind III cuts AAGCTT
between the two irst A?s. EcoRI cuts at GAATTC between the G and the
A. Hind III and EcoRI both make sticky ends.
Results:
Our results for this lab were EcoRI separated into five fragments.
Hind III separated into four fragments. The control only had one fragment.
(See chart A and figure 1-1 for distances)
Discussion:
The purpose of this lab was to see how gel electrophoresis
separates DNA fragments. We used Hind III, EcoRI, and a controlled
enzyme. Some fragments were hard to see because of smearing. These
were the bigger fragments. Loading the DNA was difficult and if you
weren?t careful you could rupture the wells which ruined the lab. We,
fortunately, did not run into this problem.
Abstract:
The purpose of this lab is to separate DNA fragments with gel
electrophoresis using EcoRI and Hind III. Restriction enzymes are used to
break up the DNA, then negatively charged DNA is placed in a gel
casting tray. Then it is placed into an electrophoresis chamber. An
electrical field is placed across the agarose gel which forces the
fragments to move down the gel. The amount of lines show how many
fragments there is in the DNA. We had five fragments for EcoRI and six
for Hind III. The no enzyme had only one fragment.
Procedures:
We sealed the ends of a gel casting tray with masking tape and
inserted a comb into the slots. The tray was filled about 6mm high with
agarose gel. It covered half the height of the comb. We waited ten
minutes for the gel to solidify. Then we placed the tray in a gel box and
made sure that the comb was at a negative (black) end. The box was
filled with tris-borate-EDTA buffer so it covered the entire surface of the
gel. The combs were removed without ripping the wells. The micro pipet
was used to load the lambda EcoRI, lambda Hind III, and lambda only
into the wells. We dipped the pipet trough the surface of the buffer over
the wells and expelled the contents. The top of the electrophoresis
chamber was closed and electrical leads were connected. The dye was
observed as it moved shortly after the power supply was turned on. The
power supply was turned off after the bands migrated near the end of
the gel and the top of the electrophoresis chamber was removed. We
removed the gel from the gel casting tray and examined it under a light
box and compared it to the ideal gel (figure1-2).
:
Restriction Enzymes: Cleavage of DNA lab
University of Illinois. (1999). Experiment 2
Gel Electrophoresis of DNA. In
Molecular Biology Cyberlab, online:
Http://www.life.uluc.edu/molbio/geldigest/electro.html
Другие работы по теме:
Melville
’s “Bartleby The Scrivener”: Introduction Of Character Essay, Research Paper Melville’s “Bartleby the Scrivener”: Introduction of Character In the first three paragraphs of ?Bartleby the Scrivener,? Melville
Cover Letter Essay Research Paper To whom
Cover Letter Essay, Research Paper To whom it may concern: I am writing to you because I am interest in a Software Engineer position. I heard about your opening through the career department at George Mason University. This position fits with my major, experience, and career interest.
The Pearl 2 Essay Research Paper IntroductionSteinbeck
The Pearl 2 Essay, Research Paper Introduction Steinbeck was born in Salinas, California, in 1902. His first 3 books were financial failures, and he worked at various kinds of jobs to The book that I read is called the Pearl and the author is Steinbesurvive, including fruit picking. His first success was Tortilla Flat (1935), which was followed by In Dubious battle a number of shorter works, leading to his great masterpiece, The Grapes of Wrath, which won the Pulitzer prize in 1940.
Aids Paper Essay Research Paper This is
Aids Paper Essay, Research Paper This is a pretty decect AIDS paper I wrote for an English class I think I got a B+—–I. Introduction Thesis-Practicing safe sex is essential in a world where diseases like AIDS exist. II. Bodya. There are many forms of safe sex, abstinence being the only 100% effective one. 1. If one chooses to have sex then condom use is very important. 2.
James Clerk Maxwell Essay Research Paper IntroductionHave
James Clerk Maxwell Essay, Research Paper Introduction Have you ever left a football outside? If you have you probably noticed it got smaller. And when you took it inside and set it next to a furnace or a vent blowing hot air, the ball tends to get a little bigger. This process can be best described by the Kinetic Molecular theory.
Small Place Essay Research Paper you need
Small Place Essay, Research Paper you need to keep track of my fucking credits so i don’t need to do thisAntigua-born Jamaica Kincaid offers a personal and opinionated introduction to the tiny island, from its natural beauty to the political corruption that leaves it without adequate hospitals and schools.
Hirosema Essay Research Paper The bombing of
Hirosema Essay, Research Paper The bombing of Hiroshima has been a highly disputed piece of history for generations. Their are very strong opinions on either side of the fence, although choosing a side is harder than meets the eye. After researching the cold facts I have humbly changed my opinion of the matter.
Aids Essay Research Paper Engl 118 April
Aids Essay, Research Paper Engl. 118 April 14, 1996 I. Introduction Thesis- Practicing safe sex is essential in a world where diseases like AIDS exist. II. Body
Proposals Essay Research Paper BackgroundThe University has
Proposals Essay, Research Paper BackgroundThe University has been looking to centralise card services for some time, recognising that the introduction of a single, multifunctional card would not only be more convenient, but also reduce the amount of work involved in managing the various cards required by different areas within the University.
How To Write An Essay 2 Essay
, Research Paper Writing an essay may seem like a huge obstacle to overcome, but with a plan of attack and a little organization, it can be easily completed. All it takes is seven easy steps.
LOTF Essay Research Paper Introduction
LOTF Essay, Research Paper Introduction The first main problem the boys have in the novel Lord of the Flies is that they are unprepared for survival on an uninhabited
Men In The Workplace Essay Research Paper
TED LEWELLEN, Professor of Anthropology at the University of Richmond, received his B.A. from Alaska Methodist University, his M.A. from New York University, and his Ph.D. from the University of Colorado. He has done field work in Peru
Edward Taylor Essay Research Paper I IntroductionA
Edward Taylor Essay, Research Paper I. Introduction A. Biographical info on author 1. born in LeiceSterchire in 1642 2. Came to New England in 1668 3. graduated from Harvard I 1671 where he studied divinity
President Clinton Essay Research Paper The content
President Clinton Essay, Research Paper The content of President Clinton’s speech was elaborated and over zealous. His focus was directed on three topics: the past, the present, and the future. In the introduction, he started by addressing the challenges that will be bestowed upon us in the next century.
Tool Essay Research Paper ESSAY OUTLINE FORMMain
Tool Essay, Research Paper ESSAY OUTLINE FORM Main (Controlling) Idea of the essay: Three main points of argument (1)(2)(3) INTRODUCTION: [Introduction should start on a general level with lead-in statements and gradually focus in on the specific topic of the essay. In the introduction, the reader should find the main idea of the essay expressed in the thesis sentence.
Printer Technology Essay Research Paper Since the
Printer Technology Essay, Research Paper Since the introduction of computers into our society, there have been countless advancements in this technology. One of the most frequently used output devices in the computer system is the printer. Like all other components of the computer, this piece of equipment has been developed and improved over time.
Cold New World Essay Research Paper Cold
Cold New World Essay, Research Paper Cold New World: Summary Just from reading the introduction I feel that “Cold New World is a perfect title for this novel. The author tells about his shift into a known, but unknown new place. I have been living in America for twenty-one years and I would have to agree with the author when he says that this country was (and is) in a strange, even an unprecedented condition.
In HOuse Pollution Essay Research Paper IntroductionThe
In HOuse Pollution Essay, Research Paper Introduction The phrase ?House? isn?t a strange thing for us. Everyone in the earth wish to has his or her own house to live in. People use their house for shelter and a place to sleep, cook, bath, relax and many more. What?s the most important is that they could spend their time with their families.
Chemistry Lab Essay Research Paper Lab ReportIntroductionThe
Chemistry Lab Essay, Research Paper Lab Report Introduction: The purpose of this lab was determining the molecular weight of an unknown solid organic compound by freezing point depression. In order to do so, one has to first, freeze cyclohxane, by putting it in ice cold water, and see what the constant temperature comes out as.
Gffdg Essay Research Paper B A B
Gffdg Essay, Research Paper B A B paper is generally focused on a more particular problem or issue; it reveals that the author has gone through brainstorming
An AllImportant Introduction Essay Research Paper In
An All-Important Introduction Essay, Research Paper In the first three paragraphs of ?Bartleby the Scrivener,? Melville introduces a character who will be played upon and defined throughout his text. This introduction is crucial to the story and the fact that the lawyer introduces himself greatly increases its effectiveness.
Hot Dog Essay Research Paper The Night
Hot Dog Essay, Research Paper The Night I Became a Convict (Well it sure seemed like it) Introduction: Police Sirens glaring ? lights flashing ? nervous and full of fright
Volcanoes Essay Research Paper My Report is
Volcanoes Essay, Research Paper My Report is on Volcanoes. For a starter, I have a lay out of the plan for my report. Also I would like to follow a volcano in it’s path. Right now here is part of my report.
Retail Trade Essay Research Paper Write a
Retail Trade Essay, Research Paper Write a structure-performance study of the retail trade industry Paper should include the ff structure 1 Introduction. In this section you give an overview of the industry, its size, growth over the past 10-20 yrs, its important to the economy and other significant factors. 1-2 pages
Medicare Essay Research Paper MEDICAREMany articles
Medicare Essay, Research Paper -MEDICARE- Many articles have been written about the medicare program in Canada. It’s a real intense topic in Canada today. This brief essay is based on the costs of medicare , an introduction to the medicare program, and if our country Canada can afford the medicare program.